Fig. 10: Suppression of the hedgehog-like signal increases resistance to mitochondrial stress but reduces fertility in mice.
From: A germline-to-soma signal triggers an age-related decline of mitochondrial stress response
a, b qRT-PCR analysis of Gli1, Hhip, and Smo mRNA levels in seven-month-old C57BL/6J male (a) and female (b) mice treated with DMSO or itraconazole. c, d qRT-PCR analysis of Chop and Asns mRNA levels in seven-month-old C57BL/6J male (c) and female (d) mice treated with DMSO or oligomycin. e, f Weight loss in seven-month-old C57BL/6J male (e) and female (f) mice during oligomycin treatment. g Litter size of seven-month-old C57BL/6J females treated with DMSO or itraconazole, mated with males of the same treatment group. h, i Serum AMH (h) or testosterone (i) levels assessed by ELISA in mice treated with DMSO or itraconazole. j–l Pearson’s correlation of GLI1, GLI2, and UPRmt mRNA levels in monkey (j), rat (k) and human (l) tissues. Blue indicates a positive correlation and white indicates a negative correlation. The intensity of the colors corresponds to the correlation coefficient. m Pearson’s correlation of GLI1, GLI2, GLI3, and human milk mRNA levels in human breast tissues. Blue indicates a positive correlation and white indicates a negative correlation. The intensity of the colors corresponds to the correlation coefficient. Error bars indicate mean ± SD. n represents the number of mice. p values were assessed using a two-tailed t test. Source data are provided as a Source Data file.
