Fig. 3: Association of CENH3 with centromeric satellite arrays.

a–b Immuno-FISH showing partial colocalization of Lusy1 repeats (magenta) and CENH3 (green) in metaphase chromosomes (counterstained with DAPI, gray). c Box plot of sizes of centromeric satellites Lusy1 and Lusy2 arrays associated with CENH3 (functional) or non-centromeric (nonfunctional). Statistical significance was tested using two-tailed Mann-Whitney U test. The number of observations in each group (array count) is indicated below individual boxes. d Abundance of dyad symmetries in functional and nonfunctional arrays of centromeric satellites. Statistical significance was tested using one-tailed Mann-Whitney U test. The number of observations (array counts) is identical to Fig. 1, panel c. e Homogeneity of functional array fragments (regions overlapping CENH3 domains) and whole nonfunctional arrays of centromeric satellites Lusy1 and Lusy2. Dot plots show sequence similarity between groups of concatenated arrays from the entire genome (left), histograms show the frequency distribution of similarity values (right). Images in a and b represent single slices of 3D-SIM image stacks. Dot plots are shown proportional to their genomic abundance. Boxplots (panel c) and inner boxes of violin plots (panel d) follow the definition in seaborn data visualization package where central lines (points in violin plot) represent median value, boxes represent 1st and 3rd quartiles, and whiskers represent the data range without outliers, defined as observations further than 1.5 of interquartile range from the respective (1st or 3rd) quartile. Source data are provided as a Source Data file.