Fig. 6: Comparison of dynamics of intratumoral immune cell populations and immune-related gene expression presented as delta (Δ) values in major and minor responders.

a–f Dynamics of cell density of immune cell populations in the tumor microenvironment assessed through multiplexed immunofluorescence represented as delta (Δ) expression values in minor pathologic responders (blue) and minor pathologic responders (green), showing post-chemo minus baseline (delta 1; minor, n = 15; major, n = 7), resection minus post-chemo (delta 2; minor, n = 12; major, n = 5), and resection minus baseline (delta 3; minor, n = 18; major, n = 5). Boxplots represent the median, and 25th and 75th percentiles; the whiskers extend from the hinge to the largest value no further than 1.5× IQR from the hinge. The difference between major and minor responders was tested using a two-sided Wilcoxon Rank-sum test. Only significant p-values are shown. a CD3 + T cells. b CD3 + CD8 + T cells. c CD3 + FOXP3 + T cells. d CD8 + /FOXP3 + T cell ratio. e CD20 + B cells. f CD68+ macrophages. Source data are provided as a Source Data file.