Fig. 5: Spatiotemporally resolved mesoscopic imaging of iAdo dynamics in head-fixed mice in vivo. | Nature Communications

Fig. 5: Spatiotemporally resolved mesoscopic imaging of iAdo dynamics in head-fixed mice in vivo.

From: A high-performance fluorescent sensor spatiotemporally reveals cell-type specific regulation of intracellular adenosine in vivo

Fig. 5

a Schematic illustration depicting the experimental design of in vivo mesoscopic imaging for (b–d). b Representative cortical expression images of neuronal expression of iAdo sensor HypnoS and membrane-targeted calcium sensor jRGECO1a (mjRGECO1a) after kainic acid (KA) injection. Two ROIs are labeled. c The response of the HypnoS and mjRGECO1a in two ROIs. The solid and dashed lines in traces correspond to ROI 1 and ROI 2 shown in b, respectively. The red shading in the EEG, EMG, and velocity trace indicates the epileptic seizure. d HypnoS and HypnoS-mut peak responses in mice during the epileptic seizure. 8 seizure events from n = 4 mice for HypnoS and 10 seizure events from 3 mice for HypnoS-mut. Two-tailed Student’s t-test: for maximum response, P = 0.0057 between HypnoS and HypnoS-mut. e Representative images of the wave propagation detected by the indicated sensor. The white circle indicates the origin of waves; small white arrows indicate the wave-propagating velocity vector; green arrows indicate example propagating trajectories. L lateral, M medial, A anterior, P posterior. f Probability distributions of wave-propagating speeds and directions calculated using indicated sensors. g Probability distributions of the rise, peak, and decay time for hSyn-HypnoS (green) and mjRGECO1a (red). The rise time is defined as the time required to achieve 50% of the maximum response, starting from the point when mjRGECO1a reaches 50% of its maximum response. Similarly, the decay time is defined as the time required to return to 50% of the maximum response in the descent phase. The peak time is defined as the time required to reach maximum responses. h Representative pseudocolor images (top) and probability distributions (bottom) of time intervals between HypnoS and mjRGECO1a in the rising phase, at peak response, and in the decaying phase. n = 3 mice for hSyn-HypnoS and mjRGECO1a. All the data are shown as mean ± s.e.m. with the error bars or shaded regions indicating the s.e.m. **P < 0.01. Source data are provided as a Source Data file.

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