Fig. 6: In vivo two-photon imaging resolves cell-type-specific iAdo dynamics.
a Left, schematic illustration depicting the experimental design of in vivo two-photon imaging in the mouse motor cortex for b–j. Right, images of neuron expressing HypnoS from −90 μm to −170 μm. b Representative images of expression and responses of HypnoS after KA or saline injection. 6 ROIs are labeled, and their traces are shown in d. f Group summary of the peak fluorescence changes of HypnoS in response to KA or saline injection. 120 ROIs are equally selected from 3 mice. Two-tailed Student’s t-test: for maximum response, P = 6.5 × 10−55, between saline and KA injections. c, e, g, Similar to b, d, f except for astrocyte expressing HypnoS. 60 ROIs are equally selected from 4 mice. Two-tailed Student’s t-test: for maximum response, P = 2.1 × 10−38, between saline and KA injections. h Representative images show neuronal (upper panels) and astrocytic (bottom panels) HypnoS expression and responses during epileptic seizure at a single cell level. i Representative responding traces of astrocytic and neuronal expressed HypnoS presented in h. j Group summary of decay time (τoff) of HypnoS expressed in astrocytes and neurons. For astrocytes, n = 60 ROIs from 3 mice; for neurons, n = 113 ROIs from 3 mice. Two-tailed Student’s t-test: for decay time, P = 2.4 × 10−37, between astrocytes and neurons. Scale bars, 25 µm (b, c) and 20 µm (h). All the data are shown as mean ± s.e.m. with the error bars or shaded regions indicating the s.e.m. ***P < 0.001. Source data are provided as a Source Data file.
