Fig. 6: Betaine-loaded LNP encapsulating mOVA induces stronger immune response. | Nature Communications

Fig. 6: Betaine-loaded LNP encapsulating mOVA induces stronger immune response.

From: Freezing induced incorporation of betaine in lipid nanoparticles enhances mRNA delivery

Fig. 6

a Schematic of vaccination regimen using C57BL/6 mice. b OVA-specific IgG response in the serum from 0.1 µg and 1 µg of mOVA-LNP vaccinated mice were analyzed by ELISA (n = 4 biologically independent samples). Data are shown as mean ± SD. c Optimal images of IFN-γ-spot-forming cells via ELISpot assay. Splenocytes were isolated 3 weeks post prime shot and stimulated by CD4 or CD8 peptide. d Quantitative analysis of IFN-γ spots of ELISpot assay stimulated with CD4 or CD8 peptide (n = 4 biologically independent samples). Data are shown as mean ± SD. Figure 6a was created in BioRender. Lu, X. (2025) https://BioRender.com/xdgkl76.

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