Fig. 2: Genetic evidence that CD200R1-CD200 suppresses phagocytosis.
From: CD200R1-CD200 checkpoint inhibits phagocytosis differently from SIRPα-CD47 to suppress tumor growth
a Flow cytometry analysis of CD200R1 expression (left) on WT (top) or Cd200r1−/− (bottom) BMDMs, and CD200 expression (right) on Cd200+/+ (top) or Cd200−/− (bottom) WEHI-231 cells, as measured by flow cytometry. Red curves, CD200R1 or CD200 mAbs. Filled curves, Ctrl mAb. b As per Fig. 1d, except that phagocytosis of IgG-opsonized WEHI-231 cells in the presence of WT or Cd200r1−/− BMDMs was studied, (n = 3). c As per Fig. 2b, except that phagocytosis of IgG-opsonized Cd200+/+ and Cd200−/− WEHI-231 cells, in the presence of WT or Cd200r1−/− BMDMs, (n = 3). Data are from three independent experiments (a–c). Each symbol represents one mouse. Data are mean ± s.e.m. Statistical analysis: two-way ANOVA test, with multiple comparisons (b, c), ns not significant. See also Supplementary Fig. 2.
