Extended Data Fig. 7: The ADCP1-HMGA recognition pair triggers the formation of chromocenter-like condensates in rice and maize protoplasts.
a, BiFC assays using ADCP1 and HMGA homologs from soybean and tobacco in Col-0 protoplasts. Scale bar, 5 μm. b, Schematic diagram of the ADCP1-HMGA pair expression in rice and maize protoplasts. The Arabidopsis, rice and maize plant models were created with BioRender.com. c,d, The distribution change of ADCP1-mCherry, HMGA-GFP, and DAPI signals in the nuclei of rice (c) and maize (d) protoplasts over time. e-g, Transformation of ADCP1 protein alone does not affect the pattern of DAPI staining in the nuclei of tobacco (e), rice (f), or maize (g). The images were taken at 72h after transformation. Scale bar, 5 μm. At least 50 independent nuclei were observed in each experiment, and the representative ones were imaged. h, Relative expression of LTR-TEs in tobacco 84h after co-transformed ADCP1-GFP and mCherry-HMGA. The NbActin3 gene was used as the internal reference. Error bars denote standard deviation (SD) of three replicates. Two-tailed t tests.
