Extended Data Fig. 1: Effects of PBRM1 loss on tumor growth.
a) Immunofluorescence staining validates the loss of PBRM1 by CRISPR mediated knockout in 786-O and HK2 cells (results representative of 2 biological replicates). b) Representative images of colony formation assay of isogenic pairs cultured in normal 10% FBS or low serum conditions (results representative of 3 biological replicates). c) PBRM1-deficient ccRCC cell lines were transduced with either empty vector (EV) control or wild-type PBRM1, cultured in soft agar for 3 weeks and assessed for colony formation potential. d) Shown are the photographs of 786-O and NCC432 tumors harvested at the end of the in vivo tumor formation studies. e) Tumor weights of NCC2112 xenograft tumors with and without PBRM1 were measured at the end of in vivo tumor formation studies (n = 6 mice per group). Data are presented as mean values + /- s.d. P-value was computed by two-tailed t-test.
