Extended Data Fig. 6: A3E and CPM do not induce the formation of fibrillar solids but increase the mesh size of IBs.
From: A condensate-hardening drug blocks RSV replication in vivo
a, HEp-2 cells infected with RSV–P–BFP for 24 h and treated with CPM (5 μM) or A3E (25 μM) for 1 h, then fixed and stained with ThS. Purified mouse PrP amyloid fibrils adsorbed on fixed cells and treated in parallel were used as positive control. IBs are shown by P–BFP fluorescence (blue) and ThS is shown in green. Representative images from two independent experiments are shown. Scale bar, 10 μm. b, c, Fluorescent dextran beads were selected based on their approximate hydrodynamic radius (Rh) and micro-injected into HEp-2 cells that were infected with RSV–P–BFP for 20–24 h and treated for 1 h with A3E (25 μM) or CPM (5 μM). Cells were imaged 2–5 min after micro-injection under a wide-field microscope. Representative images from two independent experiments are shown in c. Scale bar, 2 μm. The fluorescent dextran signal inside IBs relative to the cytoplasm was quantified as described in the Methods. Data are mean ± s.e.m. of the signal ratio from at least 25 IBs from two independent experiments. ****P < 0.0001; ns, not significant; Kruskal–Wallis test followed by Dunn’s test for multiple comparisons.
