Extended Data Fig. 3: Single-cell data analysis of intramuscular immune components of Gsdmdf/f and GsdmdCKO mice.
From: Gasdermin D-mediated metabolic crosstalk promotes tissue repair
a-c, Analysis of intramuscular immune components of Gsdmdf/f and GsdmdCKO mice. Dynamics of percentages of each cell type in Gsdmdf/f and GsdmdCKO muscles (a). Stacked violin plot of signature marker genes of each immune cell type (b) and dynamics of immune cell percentages in Gsdmdf/f and GsdmdCKO muscles (c). d, Flow cytometry analysis of myeloid cell composition at 2 dpi (n = 8). e,f, Violin plot (e) and dotplot of VISION enrichment analysis of indicated signature genesets (f). g, Dotplot of the enrichment score of indicated gene sets in highly dynamic immune cells determined in Ccr2high monocytes, Spp1high and Mrc1high macrophages.h, Trajectory analysis of the monocyte-macrophage lineage. i, Expression of the top ranked ligands in immune cells upon injury. j,k, Cell-cell interaction analysis using NicheNet. Top ranked intramuscular ligands at 2 dpi (j) and expression of top ranked receptors in each immune cell type (k). A representative of at least two independent experiments is shown. Unpaired two-tailed t test applied for (d). Mean ± SEM.
