Extended Data Fig. 2: Oestrous cycle dependent proliferation of tumour cells during oestrus stage can be reproduced in the MMTV-Wnt1 tumour model.
From: The oestrous cycle stage affects mammary tumour sensitivity to chemotherapy
a, Schematic representation of the multiday intravital microscopy setup (left) with overview images (single Z-plane) (centre) of the first imaging session (top) and the following imaging session (bottom) that are presentative for 6 experiments. Rectangles indicate example clones represented over multiple imaging days (right). Scale bar, 100 µm (overview), 50 µm (zoom). b, Clone growth rate of all quantified clones from multiday IVM imaging of transplanted MMTV-Wnt1 model. Each line represents a clone, and coloured lines highlight coordinated growth of individual clones. c, Immunofluorescent images (left) and violin plots depicting quantification (right) of EdU incorporation in the genetic MMTV-Wnt1 model representative for n = 5 (oestrus) and n = 4 (dioestrus) mice. Scale bar, 100 µm. Data is depicted as violin plots, thick solid line represents median and solid lines indicate the 25th and 75th percentiles, each dot represents a tumour (c). Statistical analysis was performed using one-sided Wilcoxon-Mann-Whitney test (c). *P < 0.05. More details on statistical analysis can be found in Supplementary File 1.
