Extended Data Fig. 4: CGRP/Ramp1 expression in GCs.
From: Nociceptive neurons promote gastric tumour progression via a CGRP–RAMP1 axis
(a) Expression levels of Calca in the stomach-innervating sensory neurons from the NGF-overexpression mice and the control mice (n = 10 mice/group). (b) Concentrations of CGRP peptide in the stomach from the NGF-overexpression mice and the control mice (n = 10 mice/group). (c) Dot plot of single-cell transcriptome data of Substance P receptors and Somatostatin receptors from mouse stomach (GSE157694 and GSE116514) and human stomach (OMIX001073). (d) UMAP of single-cell transcriptome data from mouse stomach (GSE157694). (e) Representative image of in situ hybridization of Ramp1 RNA in mouse gastric antrum (n = 5 mice/group). Scale bar, 100 μm. (f) Representative images and (g) quantification of in situ hybridization (RNAscope) of Calcrl RNA in mouse stomach and gastric cancers (n = 5 mice/group). Scale bar, 100 μm. (h) RNA levels of Ramp1 and Calcrl in YFP+ cell from Atp4b-Cre; Cdh1fl/fl; KrasG12D; Trp53fl/fl; YFP mice (n = 3 mice/group). (i) protein levels of Ramp1 and Calcrl in mouse GC cell (ACKP) and human GC cells (KATO III and AGC). (j-k) Kaplan-Meier curves of TCGA survival data. Data represent mean ± SEM, and P values were calculated by t test in a, b and g, by Logrank in j and k. The statistical tests were two-sided.
