Extended Data Fig. 5: Additional analyses of behavior and calcium imaging of vHipp D1 and D2 neuronal activity during EPM testing.
From: Dopamine D1–D2 signalling in hippocampus arbitrates approach and avoidance
Data are obtained from the same mice as in Fig. 3 (D1: n = 11, D2: n = 10 male mice). a, Distance travelled (left; t-test: t14.63 = −0.73 p = 0.4783) and velocity (right; t-test: t14.70 = −0.71 p = 0.4885). b, Open arm (OA) exploration time (left; LMM-ANOVA: cell type F1,19 = 0.0013 p = 0.9713, compartment F2,38 = 498.02 p < 0.0001, cell type x compartment F2,38 = 8.12 p = 0.0012; followed by FDR-adjusted post-hoc tests), total number of OA entries (middle; LMM-ANOVA: cell type F1,19 = 1.87 p = 0.1876, compartment F2,38 = 64.02 p < 0.0001, cell type x compartment F2,38 = 2.51 p = 0.0950; followed by FDR-adjusted post-hoc tests) and average OA exploration bout length (right; LMM-ANOVA: cell type F1,19 = 0.011 p = 0.9167, compartment F2,38 = 36.85 p < 0.0001, cell type x compartment F2,38 = 0.14 p = 0.8673; followed by FDR-adjusted post-hoc tests). c, Number of head-dips (total: t-test: t18.945 = 0.83 p = 0.4170; per behavioral outcome: LMM-ANOVA: cell type F1,19 = 0.69 p = 0.4180, outcome F2,38 = 44.21 p < 0.0001, cell type x outcome F2,38 = 4.04 p = 0.0256; followed by FDR-adjusted post-hoc tests). d, Correlation of GCaMP6s signal intensity with individual OA (left, D1: n = 163; Pearson’s r = 0.0499, p = 0.5273; D2: n = 107; Pearson’s r = 0.3242, p = 0.0007) or closed arm (CA, right, D1: n = 197; Pearson’s r = −0.2383, p = 0.0007; D2: n = 198; Pearson’s r = −0.1984, p = 0.0051) exploration bout lengths. e, Average GCaMP6s signal in D1-Cre (n = 27 head-dips) and D2-Cre (n = 36 head-dips) mice around head-dips events with unclear outcome. Boxplot represents the median time ± inter-quartile range of the times of maximal acceleration (max. acc.) after each head-dip. f, Maximum (peak) GCaMP6s signal before (pre) or after (post) each head-dip event. LMM-ANOVA: cell type F1,22.22 = 0.27 p = 0.6069, outcome F2,375.77 = 7.06 p = 0.0010, pre-post F1,385 = 84.64 p < 0.0001, cell type x outcome F2,375.77 = 16.74 p < 0.0001, cell type x pre-post F1,385 = 0.17 p = 0.6775, outcome x pre-post F2,385 = 11.69 p < 0.0001, cell type x outcome x pre-post F2,385 = 34.37 p < 0.0001; followed by FDR-adjusted post-hoc tests. g, GCaMP6s signal change slope after head-dip events. LMM-ANOVA: cell type F1,23.29 = 1.29 p = 0.2679, outcome F2,380.68 = 6.90 p = 0.0011, cell type x outcome F2,380.68 = 28.50 p < 0.0001; followed by FDR-adjusted post-hoc tests. h, Time delay between the time of maximal GCaMP6s signal change (max. slope) and the time of movement initiation (max. acc.). LMM-summary: intercept ≠ 0 t67.07 = −3.68 p = 0.0005, and LMM-ANOVA: cell type F1,23.10 = 0.0053 p = 0.9427, outcome F2,383.84 = 0.19 p = 0.8241, cell type x outcome F2,383.84 = 0.46 p = 0.6310; followed by individual LMM-summary for intercept ≠ 0 for each cell type x outcome combination and FDR adjustment of p-values. i, SVM workflow schematic. j, Classification of unclear-outcome head-dips using a SVM trained on manually-annotated avoid/explore D1 and D2 time-series. Both average GCaMP6s signal (top) and heatmaps of individual time-series (bottom) are represented for both D1 and D2 signals, split by SVM outcome prediction. Data represented as mean ± sem, regression lines with their 95% confidence intervals.
