Extended Data Fig. 4: Viral targeting of vSub.
From: Dopamine D1–D2 signalling in hippocampus arbitrates approach and avoidance
a, Representative spread, fiber placements (red lines) and representative image of AAV-DIO-GCaMP6s expression in the vSub of D1-Cre (left) and D2-Cre (right) male mice. b, Representative spread, fiber placements (red lines) and representative image of AAV-dLight-1.1, AAV-GRABDA-1h expression in the vSub. Full hemisection illustrates spread of a control AAV-GFP virus, while zoomed insets depict faint membrane expression patterns of dopamine sensors. Note the difference in GFP signal intensity with neighboring entorhinal cortex (EC). FT fiber tip. c, Representative spread and representative image of AAV-DIO-mCherry, -hM3Dq and -hM4Di expression in the vSub of D1-Cre (left) and D2-Cre (right) male mice. Placement was verified in all mice either by confocal imaging or by dissection of fresh tissue under fluorescent illumination. d, Representative spread, fiber placements (red lines) and representative image of AAV-DIO-EYFP and -ChR2 expression in the vSub of D1-Cre (left) and D2-Cre (right) male mice. vSub was primarily targeted (dark shaded areas), but unavoidable viral spread encompassed various degrees of vCA3 and vDG (light shaded areas). Only animals with notable somatic expression in neighboring entorhinal cortex (<10%) were removed from analysis. Scale bars 500 µm.
