Extended Data Fig. 5: ELF5 and AP-1 are associated with progressively closed and open chromatin regions. | Nature Genetics

Extended Data Fig. 5: ELF5 and AP-1 are associated with progressively closed and open chromatin regions.

From: Brca1 haploinsufficiency promotes early tumor onset and epigenetic alterations in a mouse model of hereditary breast cancer

Extended Data Fig. 5

a, Quantification (mean ± s.e.m.) of ELF5 IHC staining of mammary glands of WT (n = 7) and HET (n = 6) GEMMs. b, Quantification (mean ± s.e.m.) of JUN IHC staining of mammary glands of WT (n = 4) and HET (n = 3) GEMMs. c, Representative IHC staining of total JUN (as shown in Fig. 4d) and pSer73 JUN in normal mammary gland, hyperplasia, and tumor tissues. Scale bars = 100 μm. Results are representative of n = 3 WT and n = 3 HET GEMMs. d, Immunoblot of a GFP-sorted Cre-reporter murine cell line after infection with lentivirus expressing Cre alone (–), or co-expressing the dominant-negative mutant of Jun (Jun-DN), or a non-expressed version where the coding sequence of Jun-DN is missing an ATG start codon (Ctrl). Endogenous expression of Jun is partially inhibited by Jun-DN, but not by the non-expressed control, as a result of a negative feedback loop. HSP90 serves as loading control in each respective blot. Results are representative of two experiments. e, Schematic illustrating that increased chromatin accessibility in Brca1-HET AV cells allows higher sensitivity to JUN-mediated activation of target genes.

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