Extended Data Fig. 8: Basal-B cells serve as stem cells during bacteria-induced prostate inflammation.
a, The experimental timeline for labeling Basal-B cells and E. coli infection in TN-TLR mouse prostates. b,c, Flow plots showing the percentage of ZsGreen+ tdTomato− Basal-A and ZsGreen+ tdTomato+ Basal-B cells isolating from vehicle (b) and E.coli (c) treated TN-TLR mouse prostates. d, Bar graphs showing ZsGreen+ tdTomato− Basal-A cells in ZsGreen+ basal cells, and ZsGreen+ tdTomato+ Basal-B cells in ZsGreen+ basal cells of TN-TLR mouse prostates with (n = 3 mice) or without (n = 3 mice) E. coli infection. e,f, Flow plots showing the percentage of ZsGreen+ tdTomato− Basal-A-derived luminal cells and ZsGreen+ tdTomato+ Basal-B-derived luminal cells isolating from vehicle (e) and E.coli (f) treated TN-TLR mouse prostates. g, A bar graph showing the differentiation efficiency of Basal-A cells and Bas-B cells to luminal in TN-TLR mouse prostates with or without E. coli infection (n = 5 mice per group). Data are shown as the mean ± SD. Data were analyzed by unpaired two-tailed Student’s t test; ****p < 0.0001. Scale bars, 50 μm.
