Extended Data Fig. 6: Comparative NMR analyses of the BAX WT/CBI1, BAX WT/N-CBI1, and BAX C126A/CBI1 interactions. | Nature Chemical Biology

Extended Data Fig. 6: Comparative NMR analyses of the BAX WT/CBI1, BAX WT/N-CBI1, and BAX C126A/CBI1 interactions.

From: Covalent inhibition of pro-apoptotic BAX

Extended Data Fig. 6

a–c, Measured chemical shift changes of 15N-BAX WT (40 μM) upon addition of CBI1 (5:1 small molecule:protein) (a), 15N-BAX WT (40 μM) upon addition of N-CBI1 (5:1 small molecule:protein) (b), and 15N-BAX C126A (40 μM) upon addition of CBI1 (5:1 small molecule:protein) (c), plotted as a function of BAX residue number. Chemical shift changes above the 2 s.d. cutoff (significance thresholds of 0.0499, 0.0187, 0.01579 p.p.m. for a, b, c, respectively) are colored maroon and those above the 1 s.d. cutoff (significance thresholds of 0.0329, 0.0125, 0.0107 p.p.m. for a, b, c, respectively) are colored red. Residues whose cross peaks experienced prominent signal attenuation or chemical shift perturbation upon small molecule incubation are colored beige.

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