Extended Data Fig. 5: Reactivity of PEG-conjugated tryptoline acrylamides with N112C-CCNE1.
From: An allosteric cyclin E-CDK2 site mapped by paralog hopping with covalent probes
a, Structures of PEG-conjugated tryptoline acrylamide stereoprobes. b, Gel-ABPP data showing stereoselective blockade of WX-02-520 engagement of purified N112C-CCNE1 by WX-02-588-conjugate in comparison to enantiomer WX-02-589-conjugate or WX-02-520-conjugate and WX-02-521-conjugate. Purified N112C-CCNE1:CDK2 complex (1 µM) was pre-treated with the indicated concentrations of PEG-conjugated stereoprobes (2 h) followed by WX-02-520 (1 µM, 1 h) and gel-ABPP analysis. Red asterisks mark decreased WX-02-520 reactivity with N112C-CCNE1 in samples pre-treated with WX-02-588-conjugate. Data are from a single experiment representative of two independent experiments with similar results.
