Extended Data Fig. 2: Gel-ABPP of a focused library of tryptoline acrylamides for reactivity with an N112C-CCNE1:CDK2 complex.
From: An allosteric cyclin E-CDK2 site mapped by paralog hopping with covalent probes
a, Structures of alkyne-modified tryptoline acrylamides screened for reactivity with an N112C-CCNE1:CDK1 complex. b, Gel-ABPP data showing reactivity of alkyne-modified tryptoline acrylamides (5 µM, 1 h) with purified N112C-CCNE1:CDK2 or WT-CCNE1:CDK2 complexes (1 µM). Gel-ABPP analysis was performed as described in Fig. 1d. Coomassie blue signals correspond to N112C- or WT-CCNE1. Red box marks profile of WX-02-520, which reacted with N112C-CCNE1 in a stereoselective (compared to enantiomer WX-02-521) and site-specific (compared to WT-CCNE1) manner. Data are from a single experiment.
