Extended Data Fig. 8: Synthesis and characterization of synthetic standard peptides with DAQ/LDAC modifications.
From: Quantitative chemoproteomics reveals dopamine’s protective modification of Tau
a, Workflow of synthesis of the CK19 standard peptides with DAQ/LDAC modifications on Cys311. The light or heavy synthetic peptides were incubated with 1 mM LDAC at 37 °C for 2 h, and then desalted prior to LC-MS/MS analysis. Multiple forms of dopaminations were found with peptide spectra match counts listed in the table, including DA, DAQ/LDAC and DHI. b, c, Characterization of the CK19 standard peptides with DAQ/LDAC modifications by LC-MS/MS. The MS1 (b) and MS2 (c) spectra are shown, respectively, which illustrate the isotopic signatures and fragmentation patterns of the modified light (red) and heavy (blue) peptides.
