Extended Data Fig. 8: Hematopoietic stem cell (HSC) SRS differential abundance (DA) and clusters C5 and C7 peak set analysis. | Nature Immunology

Extended Data Fig. 8: Hematopoietic stem cell (HSC) SRS differential abundance (DA) and clusters C5 and C7 peak set analysis.

From: Neutrophils and emergency granulopoiesis drive immune suppression and an extreme response endotype during sepsis

Extended Data Fig. 8

a, HSC DA across SRS with UMAP showing sampled neighborhoods colored by statistically significant enrichment (spatial FDR < 0.05). b, c, Volcano plots of differentially accessible (DA) peaks (fold change > 1.5, FDR < 0.05) for HSC (b) cluster C5 vs. all other clusters and (c) cluster C7 s. all other clusters (Wilcoxon rank-sum test). d, e, Enrichment of bulk ATAC-seq profiles in differentially open peaks for clusters (d) C5 (184 peaks) and (e) C7 (718 peaks). f, g, Enrichment of transcription factor (TF) motifs of differentially open peaks for clusters (f) C5 and (g) C7. h, ChIP-seq overlap analysis for cluster C7 differentially open peaks. The Giggle score denotes a composite significance and effect size scoring (doi:10.1038/nmeth.4556). Nhood, neighborhood; SRS, sepsis response signature; FDR, false discovery rate; ChIP, chromatin immunoprecipitation.

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