Extended Data Fig. 7: Regulation of Il12rb1 and Il12rb2 genes.
a-b, ChIP-Seq analysis of HDAC1, 4 and 7 binding to Il12rb1 (a) and Il12rb2 (b) loci in mouse Th17 cells (GSE92531). Boxes highlight potential gene regulatory regions. c, Analysis of HDAC1 binding to the promoter of Il12rb1 by ChIP-qPCR using mouse CD4+ T cells. Cells from WT mice were stimulated with anti-CD3/CD28 for 5 days, incubated with DMSO or 1 μM FK506 for 30 min and restimulated with 20 nM PMA and 1 μM ionomycin for 1 hour. d, Il12rb1 and Il12rb2 mRNA levels in CD4+ T cells from WT or Stim1/2CD4Cre mice that were cultured with DMSO or 100 nM TSA for 24 hours in the presence of 10 ng/ml IL-12. e, Analysis of chromatin accessibility, T-bet binding and GATA3 binding to the Il12rb2 locus. Top two rows show ATAC-Seq data from CD4+ T cells of WT OTII (WT) and Orai1/2CD4 (Orai1fl/fl Orai2−/− Cd4Cre) OT-II mice that had been injected into TCRα−/− host mice followed by infection with the PR8-OVA strain of influenza A virus (IAV) for 8 days. The middle two rows show ATAC-Seq data of CD8+ T cells from WT mice that were left untreated or pretreated with 2 μM cyclosporin A (CsA) for 15 mins and stimulated with 10 ng/ml PMA and 0.5 μM ionomycin (iono) or left unstimulated (resting) for 2 hours (GSE93014). Bottom rows show ChIP-Seq data from naïve mouse CD4+ T cells and Th1 cells (GSE204946). Boxes highlight potential gene regulatory regions. The table in e (right) summarizes information about chromatin accessibility and TF binding to the Il12rb2 locus. Data in c-d are the means ± SEM; statistical analysis was performed by two-sided Student’s t test. ***P < 0.001; **P < 0.01; *P < 0.05.
