Extended Data Fig. 8: LZ or DZ analysis of ZsGreen− TFH and ZsGreen+ TFH cells.
(a) Another example of PPs cryostat sections from SFB + K/BxN TH17 fate-mapping mice with the same staining as indicated in Fig. 6a is shown. Arrows indicate the ZsGreen+ TFH cells. (b-c) CD4+ T cells and ZsGreen+ T cells numbers in each GC LZ or DZ were counted from the experiment in Fig. 6a. Cell number are shown (data combined from 28 PP GCs of 13 mice, 4 independent assays). (d) Non-TH17/ TFH, ZsGreen− TFH and ZsGreen+ TFH cells were sorted from PPs of SFB + K/BxN mice. Cxcr4 transcripts were determined by qRT-PCR. Data were first normalized with the HPRT housekeeping gene, and then further normalized to one of the non-TH17/ TFH samples (value set as 1); (n = 14 mice per group. In the ZsGreen+ TFH group, some samples were pooled due to small cell numbers, data combined from 2 independent assays. Each dot represents one mouse, except in the ZsGreen+ TFH group). (e) Gating strategy for non-TH17/TFH, Rorγt+ T, ZsGreen− TFH and ZsGreen+ TFH cells in K/BxN TH17 fate-mapping mice is shown. (f) Representative plots and quantitative gMFI of Bcl-6 and CD40L in splenic non-TH17/TFH, TH17, ZsGreen− TFH and ZsGreen+ TFH cells from SFB + K/BxN fate-mapping mice are shown (n = 10 mice/group, data are representative of 2 independent experiments). In Extended Data Fig. 8, data are presented as mean values ± s.e.m. Two-tailed paired t-test in b, c. One-way ANOVA with Tukey’s multiple comparisons test in d, f.
