Extended Data Fig. 1: Gut commensal SFB but not Akkermansia muciniphila (AKK) induce TFH17 cells.
(a) Cells from the popliteal lymph nodes (pLN) of both SFB− and SFB + K/BxN mice were stimulated with PMA/ionomycin for 4 h. Representative plots and quantitative data indicating the number of TFH17 cells in pLN are shown (n = 4 mice/ SFB − , and 11 mice/ SFB + , data combined from 2 independent assays). (b-c) Splenocytes from both SFB− and SFB + K/BxN mice were stimulated with PMA/ionomycin for 4 h. Representative plots of percentage and quantitative data indicating the numbers of (b) TFH1 and (c) TFH2 cells are shown (n = 5 mice/group, data from one representative experiment). (d) Splenocytes and PP cells were harvested and subjected to TH17 analysis on day 10 after AKK colonization. Representative plots and quantitative data of the percentage and number of TH17 cells are shown (n = 13 mice/ AKK − , and 18 mice/group, combined from 4 experiments). (e) Splenocytes and PP cells from both AKK− and AKK + K/BxN mice were harvested and subjected to TFH17 analysis on day 10 after AKK colonization. Representative plots and quantitative data of the percentage and number of TFH17 cells are shown (n = 13 mice/ AKK − , and 18 mice/group, combined from 4 independent assays). (f) Quantitative data show the number of splenic α4β7+ IL-17− TFH and α4β7+ TFH17 in SFB− and SFB + K/BxN mice (n = 10 mice/ SFB − , and 13 mice/ SFB + , data combined from 3 independent assays). (g) Representative plots of KikR+ and KikG+ cells in TFH17 and IL-17− TFH cells from the spleens of sham surgery KikGR.K/BxN mice are shown. As these mice never went through photoconversion surgery, all the cells should be KikG+. The few KikR+ cells established the background noise in this procedure. In the Extended Data Fig. 1, data are presented as mean values ± s.e.m., and two-tailed unpaired t-test were used in a-f.
