Extended Data Fig. 8: Complement and atherosclerosis.
From: ApoE attenuates unresolvable inflammation by complex formation with activated C1q
a, Expression microarray analyses of aortas. Heatmaps show GO terms leukocyte migration, complement activation, phagocytosis, and cellular response to lipid. 6 weeks WT (n = 3 mice); 32 weeks WT (n = 3); 6 weeks ApoE−/− (n = 3); 32 weeks ApoE−/− (n = 3). b, aorta alternative complement pathway genes (factor B, factor H, factor D) mRNA expression in 6 weeks and 32 weeks old WT and ApoE−/− mouse aortas. 6 weeks WT (n = 3 mice); 32 weeks WT (n = 3); 6 weeks ApoE−/− (n = 3); 32 weeks ApoE−/− (n = 3). c,d, plasma cholesterol and body weight. e,f blood leukocytes and percentage. For c-f, control (n =11 mice); C5 siRNA (n =12 mice). g, blood CD4+ T cells, CD8+ T cells, and B220+ B cells by flow cytometry. Control (n = 6 mice); C5 siRNA (n = 6 mice). h, super-resolution microscopy shows colocalization of C1q (green) and ApoE (red) in human atherosclerotic plaque. Representative images from at least 3 biologically independent mouse samples. Bar 5 μm. Data in b,c,d,e,f,g represent means ± s.e.m. Two-tailed Student´s t test was applied to c.d.e.f.g; one-way ANOVA with Tukey posttest was applied to b; abbreviations: WBC, white blood cells; RBC, red blood cells; PLT, platelets; LYM, lymphocytes; MO, monocytes; GRA, granulocytes. Gene names and statistics in supplementary Table 7.
