Extended Data Fig. 3: Expression of HLA class I molecules at post-transplantation relapse.

a, Heatmap representing fold expression changes in HLA class I gene transcripts (fuchsia markers), their regulators (purple markers) and accessory molecules involved in HLA class I presentation (teal markers). Transcript levels were assessed by microarrays, comparing leukemia at diagnosis with relapses after chemotherapy (CT, n = 3) or allo-HCT (allo-HCT, n = 9). Red and green indicate transcript upregulation and downregulation at relapse, respectively. Bars on the right side of the heatmap summarize mean fold changes at post-transplantation relapse. b, mRNA expression levels of HLA-A and -C measured by locus-specific qPCR in leukemia blasts pairwise collected and purified from patients at diagnosis (red dots) and at post-transplantation relapse (blue dots) (n = 7). Dots indicate values from single patients, lines indicate mean ± s.e.m. P values were calculated by a two-sided Wilcoxon matched-pairs signed rank test at 95% CI. c, HLA class I cell surface expression by leukemia blasts, assessed by immunophenotypic analysis in samples pairwise collected from discovery series patients before allo-HCT (red dots) and at post-transplantation relapse (blue dots) (n = 33). Dots indicate values from single patients, lines indicate mean ± s.e.m. P values were calculated by a two-sided Wilcoxon matched-pairs signed rank test at 95% CI.