Extended Data Fig. 3: Intrinsic defects in ONECUT1-depleted PP cells disturb launching of the β-cell program.
a,b, GSEA analysis of differentially expressed (DE) genes in HUES8 WT and ONECUT1 KO PP cells (a) as well as PDX1+/NKX6.1+ purified PP cells from HUES8 ONECUT1 KO and WT (b) using a specific gene set for pancreatic progenitors as well as genes important for endocrine development and β-cell function. c, GSEA enrichment scores contrasting HUES8 WT and KO (or trunc) at PP stage on gene expression signatures of pancreas cells obtained from a single cell RNA-seq study (GSE81547). d, Correlation of all significant differentially expressed genes (RNA-seq) and proteins (mass spectrometry, MS) in HUES8 ONECUT1 truncated (trunc) cells at the PP stage. e, Comparison of expression values for depicted genes in HUES8 edited (ONECUT1 truncated and KO) and WT PP cells. Bar graphs represent min and max values with indicated mean normalized to ONECUT1 WT cells (RNA-seq: n = 6; qPCR: WT n = 4, KO/trunc n = 4; MS: n = 3; one-way ANOVA with Tukey’s test).
