Extended Data Fig. 7: Schematic of the gating strategy for analysis of flow cytometry data. | Nature Medicine

Extended Data Fig. 7: Schematic of the gating strategy for analysis of flow cytometry data.

From: Mosunetuzumab with polatuzumab vedotin in relapsed or refractory aggressive large B cell lymphoma: a phase 1b/2 trial

Extended Data Fig. 7

Note: a) Doublets were excluded with the Singlets gate based on FSC-H and FSC-A; b) set a debris-free white blood cell (WBC) gate based on an FSC-A by SSC-A, also a sub-gate on SSC-Alo and FSC-Amid for Lymphocytes; c) On the Lymphocytes, CD4 positive cells and CD8 positive cells were gated; d) Examine expression of HLA-DR and CD69 on the gated CD4+ cells or CD8+ cells. Boundaries between positive and negative staining HLA-DR and CD69 were based on backbone only (CD4 and CD8) stained control.

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