Extended Data Fig. 4: ELI-002 2P immunization induces robust T cell responses to mKRAS antigens. | Nature Medicine

Extended Data Fig. 4: ELI-002 2P immunization induces robust T cell responses to mKRAS antigens.

From: Lymph-node-targeted, mKRAS-specific amphiphile vaccine in pancreatic and colorectal cancer: the phase 1 AMPLIFY-201 trial

Extended Data Fig. 4

Patient 11 was immunized with 1.4 mg Amph-Peptides 2P admixed with 5 mg of Amph-CpG-7909. PBMCs were collected for ex vivo T cell response assessments at baseline (B) and week 9. a, Schema showing dosing and experimental schedule. PBMCs were stimulated with OLPs to b, G12D or G12R, c, G12V, G12C, G12A, G12S or G13D or d, WT KRAS in the same IFNγ/GrB Fluorospot assay for 44 hours. Shown are background-subtracted IFNγ and/or GrB SFCs per 1 × 106 PBMCs; dotted line indicates 50 SFC/1 x 106 PBMCs. The pie charts in b, c show the percentage of mKRAS OLP-stimulated PBMCs at week 9 secreting IFNγ, GrB or both IFNγ and GrB. e, f, PBMCs were stimulated with G12R or G12D OLPs prior to analysis for intracellular cytokines, IFNγ, TNFa and IL-2 by flow cytometry. Shown are frequencies of total IL2, TNFα, IFNγ polyfunctional cytokine producing e, CD4+ and f, CD8+ T cells. The pie charts in e, f show the percentage of G12R and G12D stimulated PBMCs producing IFNγ, IL2, TNFα, producing 2 cytokines (2+) or producing 3 cytokines (3+) at week 9. g, The pie charts depict the percentage of G12R-stimulated memory CD4+ and CD8+ T cells in cytokine negative and cytokine positive cells at week 9 (Naïve: CCR7+ CD45RA+, Central memory: CCR7 + CD45RA-, Effector memory: CCR7- CD45RA-, Terminal effector memory: CCR7- CD45RA+).

Back to article page