Extended Data Fig. 1: Alternative splicing of the human p16 proximal promoter produces two distinct variants. | Nature Medicine

Extended Data Fig. 1: Alternative splicing of the human p16 proximal promoter produces two distinct variants.

From: Effects of intermittent senolytic therapy on bone metabolism in postmenopausal women: a phase 2 randomized controlled trial

Extended Data Fig. 1

Schematic representation of the proximal p16 promoter and alternative splicing patterns of the pre-mRNA, indicating the p16-variant 1 mRNA (Exons 1/2/4; NM_000077) and the p16-variant 5 mRNA (Exons 1/2/3/4; NM_001195132). The protein products between the two variants encode identical proteins from amino acids 1-152, however the p16-variant 5 protein has a unique 15 amino acid C-terminal sequence in place of the 4 amino acid C-terminal sequence of the p16-variant 1 protein, due to the retained Exon 3 in the p16-variant 5 mRNA. PCR primer sequences are indicated, demonstrating that the p16_variant 1 + 5 primer pair amplifies both variant 1 and 5 isoforms, whereas the p16_variant 5 primer pair only amplifies p16-variant 5.

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