Extended Data Fig. 1: General FACS gating strategy used for sorting blood ASC. | Nature Medicine

Extended Data Fig. 1: General FACS gating strategy used for sorting blood ASC.

From: SARS-CoV-2-specific plasma cells are not durably established in the bone marrow long-lived compartment after mRNA vaccination

Extended Data Fig. 1

PBMC were first gated for lymphocytes, singlets, and viable cells (based on their FSC/SSC and Live/Death properties). CD3 and CD14 were then used as dump markers to capture CD19+ and CD19− B cell populations. Subsequent sub-gating using CD38 versus CD27 on the IgD- fraction (of CD19+ population) allows for sorting for blood ASC (CD27hiCD38hi). See Methods for antibody panels.

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