Extended Data Fig. 3: Intraoperative sequencing and classification reporting.
A) Rapid-CNS2 methylation class predictions over 1 hour of sequencing time for 16 classifiable samples from the 18 intraoperative runs. Boxplots (top) indicate calibrated scores for correct (green) and incorrect (orange) predictions. Box plots show the median (horizontal line) and the box boundaries correspond to the 25th and 75th percentiles (interquartile range). Whiskers extend up to 1.5 times the interquartile range beyond these boundaries. Any data points outside this range are displayed individually as outliers. The number of samples represented in each box plot matches the sample counts shown in the accompanying bar plots. (bottom) Each rectangle on the bar plot indicates individual samples coloured by true methylation class or inferred methylation family (GBM). X axis indicates time in minutes. Positive Y-axis indicates correct predictions, negative Y-axis indicates incorrect predictions for the prediction. B) Prediction over time for two unclassifiable samples, not included in A). (left) did not have a true class represented in the classifier and (right) had low ( < 0.3) scores for the corresponding methylation array. Both were predicted with low confidence ( < 25%).
