Extended Data Fig. 1: PREDICT T1D microRNA profiling following in vitro human islet cell death.

Freshly isolated human islet preparations (n = 6, identified by the site and/donor ID) were exposed to different concentrations (0 mM, 1 mM, 10 mM) of a nitric oxide (NO)-donor (sodium nitroprusside; SNP). Bidirectional hierarchical clustering identified increased levels of PREDICT T1D microRNAs in supernatant with increasing concentrations of sodium nitroprusside. Data are presented as normalised Ct (cycle threshold) values, as described in methods, and represent microRNA abundance as measured by TaqMan qRT-PCR. A red colour denotes higher abundance (lower Ct-value) of microRNA in the supernatant, while white colour indicates no detectable expression (Ct-value > 39). Replicates from different experiments for the same donor are identified by the same donor ID.