Extended Data Fig. 6: Comparison of scRNAseq of ME-derived Plvap + ECs to published ECs from the mouse neurohypophysis, pituitary gland and peripheral organs.

(a) Heatmap showing the top 10 most similar cell types when analyzing the top 115 differentially expressed genes for each cEC subtype. Differentially expressed genes were determined by two-sided test in Seurat comparing each subcluster to all other astrocyte cell subclusters with min.pct=0.25 and logFC>0.6. Hypergeometric p-value was calculated and -log(FDR) values are shown. Brain cell types are highlighted in blue, while peripheral cell types are highlighted in green. (b) Table showing genes common between the top 50 differentially expressed genes in Plvap ECs, tip cells and cECs1 (calculated by two-sided Wilcoxon test in Seurat comparing each EC subtype to all other ECs with min.pct=0.25 and logFC>0.6) and marker genes of ECs from the choroid plexus from Dani et al.³¹. (c) Bar plot showing the percentage overlap of the top 50 enriched genes from Plvap ECs, tip cells and cECs 1 EC subtypes (calculated by two-sided Wilcoxon test in Seurat comparing each EC subtype to all other ECs with min.pct=0.25 and logFC>0.6) and each of the top 50 enriched genes in ECs reported from Kalucka et al.²⁰. (d) Table showing genes common between Plvap+ ECs, tip cells and cECs 2 in the organs with the highest level of overlap in (c). (e) Bar plot showing the percentage overlap of the top 50 enriched genes from Plvap ECs, tip cells and cECs 1 EC subtypes (calculated by two-sided Wilcoxon test in Seurat comparing each EC subtype to all other ECs with min.pct=0.25 and logFC>0.6) and each of the top 50 enriched genes in ECs reported from Feng et al.³⁰. (f) Table showing genes common between Plvap+ ECs, tip cells and cECs 1 in the organs with the highest level of overlap in (e). (g) Venn diagram showing the overlap between EC marker genes from the kidney and pancreas from Feng et al.³⁰ and the choroid plexus from Dani et al.³¹ with marker genes of Plvap ECs (calculated by two-sided Wilcoxon test in Seurat comparing Plvap ECs to all other ECs with min.pct=0.25 and logFC>0.6). The 9 genes common to all samples are listed in the center. (h) The top 100 genes enriched in ME-derived Plvap+ ECs when compared to cortex-derived cECs (as in (a), blue) were compared to the top 100 genes enriched in vascular ECs from the mouse neurohypophysis³² (gray) and the top 100 genes enriched in ECs isolated from the mouse pituitary gland²³ (red). Plvap+ EC enriched genes were calculated by two-sided Wilcoxon test in Seurat comparing each EC subtype to all other ECs. (i) Table containing the identities of the 8 genes expressed by all samples compared in (h).