Extended Data Fig. 3: Remodeling of adult hippocampal neurogenesis in exercise and AD.
a, Quantification of BrdU+NeuN+ adult-born neurons in the dorsal and ventral DG and representative higher magnification confocal images with anti-BrdU (green) and NeuN (red) from WT and APP/PS1, sedentary or running, mice. Scale bar, 50 µm. n = 6 per group. Two-way ANOVA, Dorsal DG: Exercise ****P < 0.0001, Genotype *P = 0.0269, Exercise x genotype n.s. P = 0.058, Ventral DG: Exercise *P < 0.0101, Genotype n.s. P = 0.1629, Exercise x genotype n.s. P = 0.305. b, Quantification of DCX+ cells in the dorsal and ventral DG from WT and APP/PS1, sedentary or running, mice. n = 6 per group. Two-way ANOVA, Dorsal DG: Exercise n.s. P = 0.0914, Genotype **P = 0.0013, Exercise x genotype n.s. P = 0.1552, Ventral DG: Exercise **P = 0.0069, Genotype ****P < 0.0001, Exercise x genotype n.s. P = 0.1426. c, Heatmap shows the normalized mean expression (z-score) of neurogenesis-related genes reported by Hochgerner et al. in our dataset. d, UMAP representations of early neuronal marker genes expression. Color represents expression level according to the scale bar on the right. e, f, Scatter plots showing the correlation between AD and exercise effects in Neuroblast I (e) and II (f). Each dot represents a statistically significant DEG in AD (WSvsAS). Dots with black borders represent statistically significant DEGs with exercise in AD mice (ASvsAR). The color gradient illustrates the recovery score (|logFC ASvsAR | ). The dot size represents the fraction of non-zero count nuclei in the AR group. g, Dot plots showing Immature Neurons’ recDEGs for all neurogenic cell types. In each, the hue and size of the dot represent the mean expression and fraction of non-zero count nuclei, respectively. Data represent the mean ± s.e.m. (a and b) of biologically independent samples.
