Extended Data Fig. 7: Degradation mechanism by NEP162.
From: Design of PROTACs utilizing the E3 ligase GID4 for targeted protein degradation
a, Analysis of BRD4 protein ubiquitination in U2OS cells pretreated with 10 µM MG132 for 12 h, followed by treatment with DMSO, 2 µM NEP108 or NEP162 for 18 h. b, Immunoblotting analysis of the degradation of natural substrate HMGCS1 in SW480 cells upon Torin1 treatment (200 nM, 16 h) in the presence of cycloheximide (50 ng/L, 16 h). c, Cell viability was conducted in U2OS cells treated with DMSO or increasing concentrations of NEP162 for the indicated times. Data are shown as mean ± S.D. (n = 3 biological replicates); two-sided t-test, *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.
