Extended Data Fig. 1: Design of GID4-based PROTACs for BRD4 degradation.
From: Design of PROTACs utilizing the E3 ligase GID4 for targeted protein degradation
a, Chemical structures of initially designed GID4-based degraders 1-4. b, RT-qPCR analysis of GID4 mRNA expression level in different cell lines. Data presented are the mean ± S.D. of three independent experiments. c, Immunoblotting analysis of BRD4 degradation in U2OS cells treated with the indicated compounds (2 µM) for 18 h. Representative images, n = 3. d, Pull-down assay using His-GID4 to pull down BRD4BD1 with addition of varying concentrations of NEP108. Representative images, n = 3.
