Figure 6

(A): Cytochalasin B administration to fetuses and whole-mount staining of 3D images at E13-24 h and E15-24 h. Green (F4/80), red (CD31), and blue (DAPI) staining. Scale bar = 100 µm. (B): Comparison between the cytochalasin B-treated and control groups after 24 h. The cytochalasin B-treated group shows a reduction in macrophage density within the wound and TNT formation. The zoom shows that macrophage cells were detected with surface mode by Imaris. Scale bar = 20 µm. (C): Comparison among the number of F4/80-positive tunneling nanotubes in the wounds. Measurements are performed at three different locations. P < 0.05. (D): Microscopic images taken 72 h after cytochalasin B administration to fetal wound sites. The administered group exhibits the disappearance of skin texture at E13-72 h, which typically undergoes complete regeneration. E15-72 h showed delayed wound healing. Scale bar = 1000 µm. E: H&E staining 72 h after cytochalasin B administration depicts delayed re-epithelialization at E15-72 h compared to the control. Scale bar = 100 µm. F: Whole-mount staining after cytochalasin B administration on E13-72 hand E15-72 h. Green (F4/80), red (CD31), and blue (DAPI) staining. Scale bar = 100 µm.