Figure 2
Effect of GL on DNA damage in human colorectal cancer cells. a γH2A.x was measured using immunofluorescence in CRC cells 24 h after GL at 300 µM. The number of γ-H2A.x per cell at the indicated time points post-GL (scale bar: 50 μm). The foci were counted in 300 CRC cells. Data from the three experiments are presented as the mean ± SEM. b The DNA tail was measured using a comet assay in CRC cells 24 h after GL at 300 µM (scale bar: 20 μm). The DNA tail per cell was counted in 100 CRC cells. Data from the three experiments are presented as the mean ± SEM. c The 8-OHdG level was measured using immunofluorescence in CRC cells at 24 h post-GL at 300 µM (scale bar: 50 μm). d The mRNA expression of ATM, RAD51, Ku70, Ku80, XRCC4 was measured by qRT‒PCR in CRC cells at 24 h post-GL at 300 µM. e Western blotting was used to detect Ku70 and LIG4 protein expression levels in CRC cells at 24 h post-GL at 300 µM. α-Tubulin was used as a loading control. *P < 0.05, **P < 0.01.
