Fig. 1
Silencing LINC01320 inhibits the growth and metastasis of OC cells both in vitro and in vivo. (A) Relative expression of LINC01320 in OC cells (SKOV3 and OVCAR3) transfected with si-LINC01320 or si-NC (negative control) tested by RT-qPCR, n = 3 per group. (B, C) The viability of OC cells was detected by CCK8 assays, n = 6 per group. (D, E) The proliferation ability of si-LINC01320-transfected OC cells was determined with a colony formation assay, n = 3 per group. (F, G) Transwell assays were performed to investigate the migratory ability of OC cells, n = 3 per group. Scale bar: 100 μm. (H–L) A total of 5 nude mice were injected subcutaneously with stably transfected OVCAR3 cells with sh-NC or sh-LINC01320. Tumors were collected and weighted (H, I). Scale bar: 1 cm. Paired Student’s t test for (I), p = 0.0358. Tumor volumes were calculated every 3 days (J). The expression of Ki-67 in tumors was evaluated with immunofluorescence (K, L). Scale bar: 20 μm. (M–O) OVCAR3 cells transfected with sh-NC or sh-LINC01320 were injected into the tails of nude mice (n = 4 per group). In both sh-NC and sh-LINC01320 mice, entire lungs were obtained (M) and nodules on the lung surfaces were counted (N). Lung sections were stained with H&E (O). For (M), scale bar: 2 mm; for (O), scale bar: 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001.
