Fig. 2
LINC01320 interacts with PURB, and PURB is required for OC cell proliferation and migration. (A) Flow chart of the RNA pull-down assays. (B) Venn diagram of proteins identified by MS from three independent RNA pull-down assays. (C) Top 10 interactors ranked by iBAQ algorithm. (D) Western blotting analysis of the expression levels of PURB in LINC01320 pull-down products. The antisense strand of LINC01320 was served as the negative control (NC). (E) RIP assays for LINC01320 binding to PURB in OC cells, with rabbit anti-IgG as the negative control, n = 3 per group. (F) qRT-PCR analysis of PURB expression in OC cells transfected with si-NC or si-PURB, n = 3 per group. (G, H) CCK-8 assays of cell viability in OC cells transfected with si-NC or si-PURB, n = 6 per group. (I, J) Colony-formation assays in OC cells transfected with si-NC or si-PURB, n = 3 per group. (K, L) Transwell assays of OC cells transfected with si-NC or si-PURB, n = 3 per group. Scale bar: 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001.
