Fig. 5
LINC01320 promotes the progression of OC by targeting /DDB2/NEDD4L/TGF-β axis. (A) OC cells transfected with si-NC, si-LINC01320, si-PURB, or si-DDB2 were analyzed by RT-qPCR for NEDD4L expression, n = 3 per group. (B–E) Western blotting was used to determine the protein expression of DDB2, NEDD4L, SMAD2, and p-SMAD2 in SKOV3 (B and D) and OVCAR3 (C and E) cell lines transfected with si-LINC01320 or si-NC, tubulin was used as an internal control, n = 3 per group. (F–K) OC cells were transfected with si-NC, si-LINC01320, si-LINC01320 + si-DDB2, or si-LINC01320 + SB431542 (TGF-β inhibitor, 30 µM). CCK-8 assays were used to verify the viability of OC cells (F, G), n = 6 per group. Colony formation assays were performed to detect proliferation ability (H, I), n = 3 per group. Transwell assays were used to investigate changes in migratory ability (J, K), n = 3 per group. Scale bar: 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001.
