Fig. 7: Expression and clinical relevance of prognostic risk model–associated key genes in NSCLC.

Using qRT-PCR to validate mRNA expression levels of TIMP1, BIRC3, and G0S2 in NSCLC tissues compared to adjacent non-cancerous tissues, with **P < 0.01, ***P < 0.001 (A-C). Quantitative analysis of TIMP1, BIRC3 and G0S2 expression in adjacent non-cancerous tissues and NSCLC tumour tissues. Statistical analysis was performed using the Student’s t-test, with ****P < 0.0001 (D–I). Kaplan-Meier survival curves for TIMP1, BIRC3 and G0S2 in NSCLC patients. Note: NA indicates not achieved (J–L). Multiplex immunofluorescence of tumour tissue (M, P) and adjacent tissue (N, Q), and CD4+, FOXP3+ and BIRC3+ ratio of tumour tissue and adjacent tissue (O, R).