Extended Data Fig. 4: NMN and NR supplementation improved kidney function and lowered inflammation in IRI mouse KD model.
From: NAD+ precursor supplementation prevents mtRNA/RIG-I-dependent inflammation during kidney injury
a, The experiment designs. NAD+ precursors (NMN or NR) or vehicle (PBS) were injected i.p. for 4 consecutive days. First dose was injected 2 h before IRI. Kidneys were collected 3 days after IRI. b, Kidney NAD+ levels in experimental groups. *P < 0.05. c, Representative images of hematoxylin and eosin staining and semi-quantitative analysis of tubule injury in experimental groups. Scale bars: 20 μm. *P < 0.05. d, Serum creatinine and blood urea nitrogen (BUN) levels in experimental groups. **P < 0.01. e, Relative expression levels of Lcn2 and Havcr1 in the kidneys of mice in experimental groups. **P < 0.01. f, Relative expression levels of Ddx58, Isg15, Irf7, ifitm3, Cxcl10, and Cxcl16 in the kidneys of mice in experimental groups. *P < 0.05, **P < 0.01. b-f, PBS n = 4. Cis + PBS n = 8. Cis + NMN n = 8. Cis + NR n = 8. Data are presented as mean ± s.e.m. and were analyzed using a one-way ANOVA followed by Tukey post hoc test for multigroup comparison.
