Extended Data Fig. 9: Expression analysis of histone acetylation, acetyl-CoA and acetyl-CoA associated enzymes with or without c-Jun knockdown after UPR^mt activation.

a, The relative band densities of Fig. 4a; left, *P = 0.037 (Control siRNA vs Control siRNA plus CDDOme), *P = 0.029 (Control siRNA plus CDDOme vs c-Jun siRNA plus CDDOme); middle, *P = 0.013 (Control siRNA vs Lonp1 siRNA), *P = 0.029 (Lonp1 siRNA vs c-Jun siRNA plus Lonp1 siRNA); right, **P = 0.0015 (Control siRNA vs Pitrm1 siRNA), *P = 0.047 (Pitrm1 siRNA vs c-Jun siRNA plus Pitrm1 siRNA); n = 3. b, Western blot analysis of H3K27Ac after UPR^mt activation with or without c-Jun knockdown at day 3 of reprogramming. c, d, Relative acetyl-CoA levels analysis after c-Jun overexpression (c) or knockdown (d) at day 3 of reprogramming; *P = 0.017 (c); *P = 0.019 (d); n = 3. e, qPCR analysis of acetyl-CoA metabolic enzymes (Acat1, Acat2 and Acot12) in CDDOme-treated or Lonp1 or Pitrm1 knockdown cells with or without c-Jun knockdown at day 3 of reprogramming; For (CDDOme plus Control siRNA) vs (CDDOme plus c-Jun siRNA), *P = 0.028 (Acat1), *P = 0.015 (Acat2), *P = 0.016 (Acot12); For (Lonp1 siRNA plus Control siRNA) vs (Lonp1 siRNA plus c-Jun siRNA), *P = 0.016 (Acat1), *P = 0.015 (Acat2), **P = 0.0048 (Acot12); For (Pitrm1 siRNA plus Control siRNA) vs (Pitrm1 siRNA plus c-Jun siRNA), **P = 0.0072 (Acat1), *P = 0.045 (Acat2), *P = 0.011 (Acot12); n = 3. f, qPCR analysis of acetyl-CoA generating enzymes (Acss2, Acly and Pdha1) and metabolic enzyme (Acaca) after UPR^mt activation with or without c-Jun knockdown at day 3 of reprogramming; n = 4. Data are mean ± SEM; * P < 0.05; ** P < 0.01. Unpaired two-tailed student’s t test (a and c-f). n represents the number of biological replicates.

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