Extended Data Fig. 10: c-Jun regulates acetyl-CoA levels by binding to the gene loci of acetyl-CoA metabolic enzymes during UPR^mt activation.

a, The relative band densities of Fig. 4c; *P = 0.018 (Control siRNA vs c-Jun siRNA); *P = 0.013 (Control siRNA vs Control siRNA plus CDDOme), *P = 0.024 (Control siRNA plus CDDOme vs c-Jun siRNA plus CDDOme); *P = 0.049 (Control siRNA vs Lonp1 siRNA), *P = 0.035 (Lonp1 siRNA vs c-Jun siRNA plus Lonp1 siRNA); **P = 0.0022 (Control siRNA vs Pitrm1 siRNA), **P = 0.0015 (Pitrm1 siRNA vs c-Jun siRNA plus Pitrm1 siRNA); n = 3. b, Relative acetyl-CoA levels in Acat1, Acat2 or Acot12 knockdown cells with c-Jun overexpression at day 3 of reprogramming; *P = 0.04 (Acat1 siRNA), *P = 0.012 (Acat2 siRNA), *P = 0.015 (Acot12 siRNA); n = 3. c, ChIP-qPCR analysis of c-Jun binding to Acat1, Acat2 and Acot12 with or without UPR^mt activation at day 3 of reprogramming; For CDDOme vs Control, *P = 0.033 (Acat1), **P = 0.0063 (Acat2), **P = 0.0018 (Acot12); For Lonp1 siRNA vs control siRNA, *P = 0.047 (Acat1), *P = 0.014 (Acat2), *P = 0.01 (Acot12); For Pitrm1 siRNA vs control siRNA, *P = 0.022 (Acat1), *P = 0.037 (Acat2), *P = 0.011 (Acot12); n = 4. Data are mean ± SEM; * P < 0.05; ** P < 0.01. Unpaired two-tailed student’s t test (a-c). n represents the number of biological replicates.

Source data