Extended Data Fig. 6: ER stress inducer tunicamycin reduces energy expenditure in young mice.
a, Downregulated gene ontology (GO) cellular component to E2 by two-tailed unpaired Student’s t-test. b, Downregulated GO reactome pathway to E2 by two-tailed unpaired Student’s t-test. c, The lean mass composition in vehicle and tunicamycin treated group (n = 5 per group). d-h, Oxygen consumption and quantification (d), ANOVA summary (Interaction P = 0.798, Treatment P < 0.0001, Time P = 0.657, by two way-ANOVA followed by Tukey’s post-hoc test), Carbone dioxygen consumption and quantification (e), ANOVA summary (Interaction P = 0.788, Treatment P < 0.0001, Time P = 0.174, by two way-ANOVA followed by Tukey’s post-hoc test), RER (f), food intake (g), and physical activity (h) (n = 7 per group). i, Quantification of basal respiration, ATP-turnover, and maximum respiratory capacity of the samples in panel (n = 7 per group). ANOVA summary (Basal; Interaction P = 0.178, PBS verse Tunicamycin P < 0.0001, Vehicle verse Estrogen P = 0.432, ATP-Linked; Interaction P = 0.505, PBS verse Tunicamycin P < 0.0001, Vehicle verse Estrogen P = 0.763, Maximum; Interaction P = 0.119, PBS verse Tunicamycin P < 0.0001, Vehicle verse Estrogen P = 0.047, by two way-ANOVA followed by Tukey’s post-hoc test). Data information: All results are presented as means ± SEM.
