Extended Data Fig. 4: Mice lacking Pnma1 or Pnma4 exhibit normal neurobehavioral and muscular traits.
From: The retrotransposon-derived capsid genes PNMA1 and PNMA4 maintain reproductive capacity
(a-g) Six-month-old male wild type (N = 11), Pnma1−/− (N = 11), Pnma4−/− (N = 8), and Pnma1−/− Pnma4−/− double mutant mice (N = 11) were tested in a panel of behavioral assays. (a) Short-term memory was assessed using the spontaneous alternation percentage in a Y maze. (b) Long-term memory was assessed with the fear conditioning test. (c-e) The open field test was used to assess indicators of anxiety (c, center time) and hyperactivity (d, distance travelled and e, vertical counts). (f) We assessed muscle strength by measuring inverted hang time on a grid and (g) grip strength force. All graphs indicate mean +/− SEM. Statistical significance was assessed by multiple t-test analysis with correction for multiple comparisons or one-way ANOVA with correction for multiple comparisons (n.s. p > 0.05, * p ≤ 0.05, ** p ≤ 0.01).
