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Sequencing refers to the techniques used to determine the primary structure of an unbranched biopolymer (DNA, RNA, protein, carbohydrate) resulting in a symbolic linear depiction of the monomeric subunits which summarizes much of the atomic-level structure of the molecule.
In this Tools of the Trade article, Jianheng Fox Liu describes CROWN-seq, a method for mapping Am (2′-O-methyladenosine), m6Am (N6,2′-O-dimethyladenosine) and transcription start sites.
Advances in single-cell multi-omics assays that co-detect two or more modalities enable a new frontier in resolving cellular heterogeneity and gene regulation.
In this Journal Club, Diyendo Massilani recalls two studies by Meyer et al. that reported a mitochondrial genome and nuclear DNA sequences from mid-Ice Age Sima de los Huesos hominins.
In this Tools of the Trade article, Loic Binan explains Perturb-FISH, which measures genetic perturbations and gene expression in situ at high throughput to map gene regulatory networks at cellular and tissue scale.
In this Tools of the Trade article, Nadiya Khyzha describes SLAM-RT&Tag, a method for profiling RNA localization and dynamics within nuclear compartments, such as speckles.
